2ELB
Crystal Structure of the BAR-PH domain of human APPL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.568, 104.126, 37.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.840 - 2.600 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.32700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.067 | 0.339 |
Number of reflections | 22741 | |
<I/σ(I)> | 27.5853 | 5.94 |
Completeness [%] | 98.3 | 98.4 |
Redundancy | 6.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES (pH 7.5), 22% (w/v) polyacrylic acid 5100, 20mM magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |