2EJW

Homoserine Dehydrogenase from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	165.439, 173.603, 97.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.360 - 1.700
R-factor	0.21
Rwork	0.210
R-free	0.23800
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	152831
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG3000, Mg(NO3)2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K