2EJV

Crystal structure of threonine 3-dehydrogenase complexed with NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-09-22
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	135.758, 135.758, 268.593
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 2.550
R-factor	0.207
Rwork	0.207
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dq4
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550
Number of reflections	48510
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.8M Sodium Acetate, 0.1M Mes pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K