2EJR

LSD1-tranylcypromine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	185.759, 185.759, 108.742
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.100 - 2.700
R-factor	0.2042
Rwork	0.204
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dw4
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.687
Number of reflections	30564
<I/σ(I)>	25.7	2.1
Completeness [%]	99.0	91.4
Redundancy	19.7	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	3.5-4% PEG 2000, 0.1M HEPES-Na, 5% MPD, 5mM Tranylcypromine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K