2EJA

Crystal Structure Of Uroporphyrinogen Decarboxylase From Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-01-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	65.473, 80.403, 128.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.790 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2inf
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.800	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.061	0.255
Number of reflections	50375
<I/σ(I)>	21.4	8.1
Completeness [%]	92.6	63.4
Redundancy	5.9	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.5	295	50v/v(%) PEG 200, 0.1M Acetic acid, pH 4.5, microbatch, temperature 295K