2EJ0

Crystal Structure of T.th.HB8 Branched-Chain Amino Acid Aminotransferase with Pyridoxamine 5'-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	115.880, 143.690, 146.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 1.600
R-factor	0.179
Rwork	0.179
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wrv
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	320144
Completeness [%]	99.3	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	NaCl, MPD, Na Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K