2EHO

Crystal structure of human GINS complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	93
Detector technology	CCD
Collection date	2006-09-20
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	164.935, 176.995, 126.508
Unit cell angles	90.00, 105.91, 90.00

Refinement procedure

Resolution	29.900 - 3.000
R-factor	0.221
Rwork	0.221
R-free	0.27400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.810	3.000
Rmerge		0.302
Number of reflections	77549
<I/σ(I)>	18	3
Completeness [%]	98.9	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1M Tris HCl, 1.8M ammonium sulfate, 0.2M lithium sulfate, 1.5% 1,2,3,-heptanetriol , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1M Tris HCl, 1.8M ammonium sulfate, 0.2M lithium sulfate, 1.5% 1,2,3,-heptanetriol , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K