2EHH
Crystal structure of dihydrodipicolinate synthase from aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-11-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.321, 146.953, 97.877 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.091 | 0.465 |
| Number of reflections | 126980 | |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 5.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 291 | 0.4M NaH2PO4, 1.6M K2HPO4, imidazole, 0.2M NaCl, pH 8.0, LIQUID DIFFUSION, temperature 291K |
