2EH8
Crystal structure of the complex of humanized KR127 fab and PRES1 peptide epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.340, 61.270, 83.540 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 32.910 - 2.600 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eh7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.910 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | |
| Number of reflections | 14611 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 100.0 | 96.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 17% PEG 4000, 10mM Hepes-NaOH, 0.2M ammonium sulfate, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
