2EFV
Crystal Structure of a Hypothetical Protein(MJ0366) from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-11-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97884, 0.9000, 0.97973 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.846, 78.846, 35.104 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.710 - 1.900 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.067 | 0.400 |
Number of reflections | 16670 | |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 12.7 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 7.5 | 293 | 2% PEG400, 2M Ammonium Sulfate, 0.1M HEPES pH7.5, LIQUID DIFFUSION, temperature 293K |