2EFV

Crystal Structure of a Hypothetical Protein(MJ0366) from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	180
Detector technology	CCD
Collection date	2006-11-16
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97884, 0.9000, 0.97973
Spacegroup name	P 43 21 2
Unit cell lengths	78.846, 78.846, 35.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 1.900
R-factor	0.219
Rwork	0.219
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.067	0.400
Number of reflections	16670
Completeness [%]	99.8	98.8
Redundancy	12.7	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	7.5	293	2% PEG400, 2M Ammonium Sulfate, 0.1M HEPES pH7.5, LIQUID DIFFUSION, temperature 293K