2EFU

The crystal structure of D-amino acid amidase from Ochrobactrum anthropi SV3 complexed with L-phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-24
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	77.468, 123.274, 116.156
Unit cell angles	90.00, 104.05, 90.00

Refinement procedure

Resolution	47.670 - 2.300
R-factor	0.17373
Rwork	0.170
R-free	0.23524
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DAA native structure (2DRW)
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.093	0.359
Number of reflections	93589
Completeness [%]	100.0	100
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1