2EFS
Crystal structure of the C-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 2.0 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-10-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.199, 63.723, 73.258 |
| Unit cell angles | 66.64, 89.99, 90.30 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.30400 |
| Structure solution method | MR |
| Starting model (for MR) | 2d3e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMOLE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.038 | 0.110 |
| Number of reflections | 47224 | |
| <I/σ(I)> | 21 | 4.8 |
| Completeness [%] | 97.4 | 93.6 |
| Redundancy | 3.87 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 40% MPD, 0.4M AMMONIUM ACETATE 0.1M TRIS-HCL, pH 8.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
