2EFC
Ara7-GDP/AtVps9a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.377, 58.474, 68.714 |
| Unit cell angles | 81.68, 86.89, 72.97 |
Refinement procedure
| Resolution | 68.040 - 2.090 |
| R-factor | 0.22409 |
| Rwork | 0.221 |
| R-free | 0.27419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | nucleotide-free Ara7/AtVps9a which was solved by MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.072 | 0.295 |
| Number of reflections | 48167 | |
| <I/σ(I)> | 10.6 | 2.3 |
| Completeness [%] | 95.1 | 78.9 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG3350, 200mM lithium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
