Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.593, 51.164, 101.135 |
Unit cell angles | 90.00, 97.53, 90.00 |
Refinement procedure
Resolution | 45.570 - 2.400 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yh3 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 21501 | |
<I/σ(I)> | 17.9641 | 4.62687 |
Completeness [%] | 93.9 | 81.4 |
Redundancy | 3.08246 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 10000, 0.1M HEPES, 0.1M SODIUM IODIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |