2EEP
Prolyl Tripeptidyl Aminopeptidase Complexed with an Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 150.103, 150.103, 160.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2d5l |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | |
| Number of reflections | 54747 | |
| <I/σ(I)> | 79.2 | 14 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.7 | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.1M Potassium Sodium tartrate, 0.2M Lithium Sulfate, 0.1M CHES Buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
