2EC8
Crystal structure of the exctracellular domain of the receptor tyrosine kinase, Kit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1000 |
Spacegroup name | H 3 |
Unit cell lengths | 162.249, 162.249, 67.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.070 - 3.000 |
R-factor | 0.25638 |
Rwork | 0.254 |
R-free | 0.29505 |
Structure solution method | MIRAS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.848 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.076 | 0.170 |
Number of reflections | 13342 | |
<I/σ(I)> | 11.7 | 7.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 277 | 12% PEG 400, 0.2M KCl, 0.1M Na-Pi buffer, pH 6.0, VAPOR DIFFUSION, temperature 277K |