2EAB
Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-06-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.183, 112.035, 98.317 |
Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
Resolution | 35.050 - 1.120 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.18900 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.168 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.160 |
High resolution limit [Å] | 1.120 | 1.120 |
Rmerge | 0.055 | 0.369 |
Number of reflections | 643080 | |
<I/σ(I)> | 30 | 2.5 |
Completeness [%] | 91.1 | 77.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M TRIS (PH 7.5), 10% ISOPROPANOL, 10% PEG6000, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M TRIS (PH 7.5), 10% ISOPROPANOL, 10% PEG6000, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 289K |