2E9X

The crystal structure of human GINS core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	57.928, 88.741, 102.129
Unit cell angles	104.69, 102.96, 95.47

Refinement procedure

Resolution	29.820 - 2.300
R-factor	0.199
Rwork	0.197
R-free	0.23300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.990	1.990
Number of reflections	101605
<I/σ(I)>	21.01	3.1
Completeness [%]	78.4	28.2
Redundancy	3.8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	0.1M Na malonate-HCl, 1M LiSO4, 0.5M (NH4)2SO4, 20mM DTT, 0.1%(v/v) isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	0.1M Na malonate-HCl, 1M LiSO4, 0.5M (NH4)2SO4, 20mM DTT, 0.1%(v/v) isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	0.1M Na malonate-HCl, 1M LiSO4, 0.5M (NH4)2SO4, 20mM DTT, 0.1%(v/v) isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	0.1M Na malonate-HCl, 1M LiSO4, 0.5M (NH4)2SO4, 20mM DTT, 0.1%(v/v) isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K