2E9F
Crystal Structure of T.th.HB8 Argininosuccinate lyase complexed with L-Arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.336, 119.787, 257.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.290 - 2.800 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aos |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.920 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 51942 | |
Completeness [%] | 85.7 | 74.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7.5% PEG 4000, 0.1M MES pH 6.5, 30% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K |