2E81
Cytochrome c Nitrite Reductase from Wolinella succinogenes with bound intermediate hydroxylamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 119.891, 119.891, 186.224 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
Rwork | 0.186 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fs7 |
RMSD bond length | 0.006 |
RMSD bond angle | 2.259 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.488 |
Number of reflections | 45781 | |
<I/σ(I)> | 12.8 | 2 |
Completeness [%] | 99.5 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 12% PEG 4000, 0.2M ammonium sulfate, 0.015M yttrium chloride, 0.1M sodium acetate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |