2E7P

Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-10-29
Detector	BRUKER DIP-6040B
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	97.774, 97.774, 91.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.750 - 2.100
R-factor	0.18591
Rwork	0.184
R-free	0.22039
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.345
Number of reflections	28968
Completeness [%]	100.0	100
Redundancy	11.4	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	20% PEG6000, 0.1M CITRIC ACID, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5	277	20% PEG6000, 0.1M CITRIC ACID, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5	277	20% PEG6000, 0.1M CITRIC ACID, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K