2E3P
Crystal structure of CERT START domain in complex with C16-cearmide (P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.976, 49.699, 62.365 |
| Unit cell angles | 77.72, 71.06, 82.10 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.030 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 92503 | |
| Completeness [%] | 92.7 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP |
