2E3K
Crystal structure of the human Brd2 second bromodomain in complexed with the acetylated histone H4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-05-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.828, 128.591, 45.847 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.20447 |
| Rwork | 0.201 |
| R-free | 0.26757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dvv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.048 | 0.166 |
| Number of reflections | 22434 | |
| Completeness [%] | 97.7 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG4000, Ammonium Acetate,, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
