2E3H
Crystal structure of the CLIP-170 CAP-Gly domain 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-08 |
| Detector | MAC Science DIP-2040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.937, 54.937, 57.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.670 - 1.450 |
| R-factor | 0.19281 |
| Rwork | 0.192 |
| R-free | 0.21483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lpl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.356 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 17953 | |
| <I/σ(I)> | 44.3 | 11.5 |
| Completeness [%] | 98.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
