1LPL

Structural Genomics of Caenorhabditis elegans: CAP-Gly domain of F53F4.3

Summary for 1LPL

• Entry DOI: 10.2210/pdb1lpl/pdb
• Descriptor: Hypothetical 25.4 kDa protein F53F4.3 in chromosome V (2 entities in total)
• Functional Keywords: structural genomics, cap-gly domain, cytoskeleton, tubulin, psi, protein structure initiative, southeast collaboratory for structural genomics, secsg, unknown function
• Biological source: Caenorhabditis elegans
• Cellular location: Cytoplasm (By similarity): Q20728
• Total number of polymer chains: 1
• Total formula weight: 10358.67

Authors

Li, S.,Finley, J.,Liu, Z.-J.,Qiu, S.H.,Luan, C.H.,Carson, M.,Tsao, J.,Johnson, D.,Lin, G.,Zhao, J.,Thomas, W.,Nagy, L.A.,Sha, B.,DeLucas, L.J.,Wang, B.-C.,Luo, M.,Southeast Collaboratory for Structural Genomics (SECSG) (deposition date: 2002-05-08, release date: 2002-05-22, Last modification date: 2024-02-14)

Primary citation

Li, S.,Finley, J.,Liu, Z.-J.,Qiu, S.H.,Luan, C.H.,Carson, M.,Tsao, J.,Johnson, D.,Lin, G.,Zhao, J.,Thomas, W.,Nagy, L.A.,Sha, B.,DeLucas, L.J.,Wang, B.-C.,Luo, M.
Crystal Structure of the Cytoskeleton-associated Protein Glycine-rich (CAP-Gly) Domain
J.Biol.Chem., 277:48596-48601, 2002

PubMed Abstract: Cytoskeleton-associated proteins (CAPs) are involved in the organization of microtubules and transportation of vesicles and organelles along the cytoskeletal network. A conserved motif, CAP-Gly, has been identified in a number of CAPs, including CLIP-170 and dynactins. The crystal structure of the CAP-Gly domain of Caenorhabditis elegans F53F4.3 protein, solved by single wavelength sulfur-anomalous phasing, revealed a novel protein fold containing three beta-sheets. The most conserved sequence, GKNDG, is located in two consecutive sharp turns on the surface, forming the entrance to a groove. Residues in the groove are highly conserved as measured from the information content of the aligned sequences. The C-terminal tail of another molecule in the crystal is bound in this groove.

PubMed: 12221106
DOI: 10.1074/jbc.M208512200

Experimental method

X-RAY DIFFRACTION (1.77 Å)