2E32
Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-29 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.942, 109.816, 153.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.530 - 3.520 |
| R-factor | 0.22651 |
| Rwork | 0.223 |
| R-free | 0.29855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e31 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.078 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 152.500 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.103 | 0.225 |
| Number of reflections | 12279 | |
| <I/σ(I)> | 7 | 4.1 |
| Completeness [%] | 85.2 | 79 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 2.0M ammonium sulphate, 0.1M sodium citrate, 30mM chitobiose, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
