2E31
Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-09 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 106.408, 106.408, 109.854 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.220 - 2.400 |
| R-factor | 0.23605 |
| Rwork | 0.233 |
| R-free | 0.29136 |
| Structure solution method | SIR-AS, MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LDK and 1UMH |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.800 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.045 | 0.327 |
| Number of reflections | 27964 | |
| <I/σ(I)> | 18.1 | 2.7 |
| Completeness [%] | 97.7 | 93 |
| Redundancy | 5.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 2.0M ammonium sulphate, 0.1M sodium citrate, 30mM chitobiose, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
