2E2Y
Crystal Structure of F43W/H64D/V68I Myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.651, 90.651, 45.447 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.340 - 1.600 |
| R-factor | 0.16959 |
| Rwork | 0.168 |
| R-free | 0.19543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lue |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.299 |
| Number of reflections | 28285 | |
| <I/σ(I)> | 23.7 | 10.7 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 11.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 2.2M Ammonium Sulfate, 20mM Tris/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
