2DYP

Crystal Structure of LILRB2(LIR2/ILT4/CD85d) complexed with HLA-G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	81.400, 81.400, 186.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.660 - 2.500
Rwork	0.233
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d31 2gw5
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.052	0.412
Number of reflections	25468
<I/σ(I)>	14.9	3.5
Completeness [%]	99.3	99.4
Redundancy	10.8	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl pH8.0, 45% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293K