2DYM
The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-46) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.347, 104.417, 112.153 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.25000 |
| Structure solution method | MIR, MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.306 |
| Number of reflections | 88015 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 98.6 | 95.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293 | 24% PEG 3350, 0.1M CAPS, pH 10, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
