2DVW

Structure of the Oncoprotein Gankyrin in Complex with S6 ATPase of the 26S Proteasome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	120
Detector technology	CCD
Collection date	2004-12-20
Detector	RIGAKU JUPITER 210
Spacegroup name	P 21 21 21
Unit cell lengths	45.398, 67.886, 107.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.169
Rwork	0.169
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	gankyrin(PDB entry:1IXV)
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.069	0.182
Number of reflections	14844
Completeness [%]	96.5	83.9
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1