2DVV
Crystal structure of the second bromodomain of the human Brd2 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-12-12 |
Detector | RIGAKU JUPITER 210 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.782, 52.604, 32.059 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BRD2-BD1(PDB entry:1X0J) |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 | 0.238 |
Number of reflections | 11743 | |
Completeness [%] | 99.5 | 95.2 |
Redundancy | 6.8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 298 | PEG4000, AMMONIUM ACETATE, PH 6.5, VAPOR DIFFUSION, TEMPERATURE 298K |