2DVV

Crystal structure of the second bromodomain of the human Brd2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	120
Detector technology	CCD
Collection date	2004-12-12
Detector	RIGAKU JUPITER 210
Spacegroup name	P 21 21 2
Unit cell lengths	71.782, 52.604, 32.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.188
Rwork	0.188
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BRD2-BD1(PDB entry:1X0J)
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.063	0.238
Number of reflections	11743
Completeness [%]	99.5	95.2
Redundancy	6.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	298	PEG4000, AMMONIUM ACETATE, PH 6.5, VAPOR DIFFUSION, TEMPERATURE 298K