2DSU
Binding of chitin-like polysaccharide to protective signalling factor: Crystal structure of the complex formed between signalling protein from sheep (SPS-40) with a tetrasaccharide at 2.2 A resolution
Replaces: 2AO8Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.750, 66.510, 107.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.000 - 2.200 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dpe |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 23000 | |
<I/σ(I)> | 14.7 | 2.6 |
Completeness [%] | 95.8 | 93.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25MM TRIS-HCL, 50MM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |