2DSF
Structure of the complex of C-terminal lobe of bovine lactoferrin with xylose at 2.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.450, 50.440, 65.870 |
| Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b17 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SORTRF |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 9236 | |
| Completeness [%] | 92.9 | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | PEG 3350, HEPES, ZnSO4, pH 6.5, VAPOR DIFFUSION, temperature 298K |
