2DRZ

Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-09
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	40.186, 89.296, 41.642
Unit cell angles	90.00, 116.31, 90.00

Refinement procedure

Resolution	27.410 - 2.190
R-factor	0.215
Rwork	0.215
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2D12
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.190	2.190
Number of reflections	12268
Completeness [%]	90.7	87.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	30% PEGMME2000, 0.2M Ammonium Sulfate, 0.1M Sodium Acetate trihydrate, 15mg/ml lactose, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K