2DR6
Crystal structure of a multidrug transporter reveal a functionally rotating mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-04 |
Detector | MACSCIENCE |
Wavelength(s) | 0.9000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 227.046, 134.561, 161.697 |
Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.300 |
R-factor | 0.302 |
Rwork | 0.298 |
R-free | 0.35900 |
Structure solution method | DIFFERENCE FOURIER |
RMSD bond length | 0.042 |
RMSD bond angle | 3.688 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | FFTBIG |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.077 | 0.334 |
Number of reflections | 69300 | |
<I/σ(I)> | 12.2 | 1.8 |
Completeness [%] | 93.5 | 84.2 |
Redundancy | 7.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | PEG4000, PH 6.1, VAPOR DIFFUSION, SITTING DROP |