2DNS
The crystal structure of D-amino acid amidase from Ochrobactrum anthropi SV3 complexed with D-Phenylalanine
Replaces: 2DD0Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-08 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.067, 123.702, 116.046 |
| Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.400 |
| R-factor | 0.19917 |
| Rwork | 0.195 |
| R-free | 0.26873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2D83 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.262 |
| Data reduction software | JUPITOR210 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.486 |
| Number of reflections | 80614 | |
| <I/σ(I)> | 0.434 | |
| Completeness [%] | 99.9 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
