2DLC
Crystal structure of the ternary complex of yeast tyrosyl-tRNA synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9798, 0.9801, 0.9819, 0.9727 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.854, 63.854, 330.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.24707 |
| Rwork | 0.245 |
| R-free | 0.28889 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.124 |
| Data reduction software | MOSFLM |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 27897 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Tris, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Tris | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | H2O |
