2DHH
Crystal structure of a multidrug transporter reveal a functionally rotating mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.870, 134.418, 163.192 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.30700 |
| Structure solution method | MIR |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.076 | 0.359 |
| Number of reflections | 117661 | |
| <I/σ(I)> | 35.3 | 2.3 |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 17.3 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | PEG4000, PH 6.1, VAPOR DIFFUSION, SITTING DROP |
