2DFD
Crystal Structure of Human Malate Dehydrogenase Type 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99806 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.943, 152.526, 154.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.010 - 1.900 |
| R-factor | 0.15774 |
| Rwork | 0.156 |
| R-free | 0.19416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mld |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.010 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 112689 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MMT, 30% PEG1000, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
