2DF7

Crystal structure of infectious bursal disease virus VP2 subviral particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 3
Unit cell lengths	316.412, 316.412, 316.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.168
Rwork	0.165
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wcd
RMSD bond length	0.016
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.096	0.578
Number of reflections	318732
<I/σ(I)>	18.6	2.1
Completeness [%]	99.5	96
Redundancy	10.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K