2DEM
Crystal structure of Uracil-DNA glycosylase in complex with AP:A containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-12 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.920, 150.170, 93.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.030 - 1.950 |
Rwork | 0.200 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ddg |
RMSD bond length | 0.025 |
RMSD bond angle | 2.300 |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.030 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.062 | 0.305 |
Number of reflections | 35480 | |
<I/σ(I)> | 12.3 | 4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.14 | 4.06 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293 | 1.4M NaKPO4, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaKPO4 | ||
2 | 1 | 1 | HOH | ||
3 | 1 | 2 | NaKPO4 | ||
4 | 1 | 2 | HOH |