2DEJ
Crystal Structure of galaktokinase from Pyrococcus horikoshii with AMP-PN and galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-16 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.945, 38.997, 81.188 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.18909 |
| Rwork | 0.187 |
| R-free | 0.22807 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2cz9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.294 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.077 | 0.478 |
| Number of reflections | 53991 | |
| <I/σ(I)> | 14.4 | 2.3 |
| Completeness [%] | 98.5 | 96.6 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 295 | 2.5% PEG 4000, 50mM magnesium chloride, 0.1M MES, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
