2DDG
Crystal structure of uracil-DNA glycosylase in complex with AP:G containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-11 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.927, 150.174, 93.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.160 - 2.100 |
Rwork | 0.218 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.043 |
RMSD bond angle | 3.100 |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.160 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.068 | 0.273 |
Number of reflections | 28574 | |
<I/σ(I)> | 9.9 | 3.7 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293 | 1.4M NaKPO4, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaKPO4 | ||
2 | 1 | 1 | HOH | ||
3 | 1 | 2 | NaKPO4 | ||
4 | 1 | 2 | HOH |