2DBT
Crystal structure of chitinase C from Streptomyces griseus HUT6037
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-01-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 153.116, 153.116, 90.031 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.320 - 3.140 |
| R-factor | 0.17625 |
| Rwork | 0.174 |
| R-free | 0.22524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wvv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.316 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.320 | 3.221 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.074 | 0.247 |
| Number of reflections | 20454 | |
| <I/σ(I)> | 9.8 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.3M Ammonium formate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
