2DBQ
Crystal Structure of Glyoxylate Reductase (PH0597) from Pyrococcus horikoshii OT3, Complexed with NADP (I41)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97892 |
| Spacegroup name | I 41 |
| Unit cell lengths | 114.252, 114.252, 119.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.290 - 1.700 |
| Rwork | 0.131 |
| R-free | 0.14290 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.392 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 84158 | |
| <I/σ(I)> | 21.94 | 4.697 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
