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2DB3

Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyCCD
Collection date2002-02-22
DetectorMARRESEARCH
Wavelength(s)0.912
Spacegroup nameP 1 21 1
Unit cell lengths71.050, 142.331, 130.465
Unit cell angles90.00, 90.86, 90.00
Refinement procedure
Resolution39.460 - 2.200
R-factor0.197
Rwork0.197
R-free0.25000
Structure solution methodMAD
RMSD bond length0.008
RMSD bond angle1.500
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0002.340
High resolution limit [Å]2.2002.200
Number of reflections117511
<I/σ(I)>16.8
Completeness [%]89.679.2
Redundancy2.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52770.1M ADA pH 6.5, 0.1M Li2SO4, 1-2% isopropyl alcohol, 69mM Cymal-3, 9-12% PEG4000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1VAPOR DIFFUSION, HANGING DROP6.52770.1M ADA pH 6.5, 0.1M Li2SO4, 1-2% isopropyl alcohol, 69mM Cymal-3, 9-12% PEG4000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111ADA
1012H2O
211Li2SO4
311isopropyl alcohol
411Cymal-3
511PEG4000
611H2O
712ADA
812Li2SO4
912PEG4000

227111

PDB entries from 2024-11-06

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