2DB3
Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.050, 142.331, 130.465 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 39.460 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 117511 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 89.6 | 79.2 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M ADA pH 6.5, 0.1M Li2SO4, 1-2% isopropyl alcohol, 69mM Cymal-3, 9-12% PEG4000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M ADA pH 6.5, 0.1M Li2SO4, 1-2% isopropyl alcohol, 69mM Cymal-3, 9-12% PEG4000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ADA | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | isopropyl alcohol | ||
| 4 | 1 | 1 | Cymal-3 | ||
| 5 | 1 | 1 | PEG4000 | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ADA | ||
| 8 | 1 | 2 | Li2SO4 | ||
| 9 | 1 | 2 | PEG4000 |
