2D5V
Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97799 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 73.020, 38.958, 106.500 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39224 | |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.2 | 293 | 1,6-hexanediol, sodium acetate, cobalt chloride, pH 5.2, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 1,6-hexanediol | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | cobalt chloride | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | cobalt chloride | ||
| 7 | 1 | 2 | H2O |
