2D57

Double layered 2D crystal structure of AQUAPORIN-4 (AQP4M23) at 3.2 a resolution by electron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Temperature [K]	4.2
Detector technology	CCD
Collection date	2002-04-01
Detector	GATAN ULTRASCAN
Wavelength(s)	0.01968
Spacegroup name	P 4 21 2
Unit cell lengths	69.000, 69.000, 160.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.210 - 3.200
R-factor	0.283
Rwork	0.283
R-free	0.33800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j4n
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MRC
Data scaling software	MRC
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.210	3.400
High resolution limit [Å]	3.200	3.200
Rmerge	0.223	0.445
Number of reflections	5992
Completeness [%]	87.0	85.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	dialysys	6	293	10mM MES(pH 6.0), 100mM NaCl, 50mM MgCl2, 2mM DTT, 1% glycerol, dialysys, temperature 293K