2D4H
Crystal-structure of the N-terminal large GTPase Domain of human Guanylate Binding protein 1 (hGBP1) in complex with GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-11 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.921, 173.450, 49.632 |
Unit cell angles | 90.00, 110.85, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.900 |
R-factor | 0.235 |
Rwork | 0.233 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2B8W |
RMSD bond length | 0.006 |
RMSD bond angle | 0.964 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 14479 | |
Completeness [%] | 97.2 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 298 | 20% Peg 3350, 130 mM NH4NO3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |